Polycyclic Aromatic Hydrocarbon (PAHs) Analyses in Marine Tissues Using Accelerated Solvent Extraction (ASE) in Tandem with In-Cell Purification and GC‑MS

The aim of this research was the replacement conventional sample extraction techniques for polycyclic aromatic hydrocarbons (PAH) in tissue samples a reliable, fast and ecofriendly procedure. method developed using pressurized solvent assessing two different standard reference materials (fish mussel) freeze-dried fortified sardine (Sardinella sp.). Five procedures were evaluated best performance comprised 1 g lyophilized tissue, 5 deactivated (5%) silica, dichloromethane:methanol (4:1 v/v) mixture, temperature 80 °C, three cycles, 10 min static time 90 s purge time. selected following these tests further validated through analysis nine replicates National Institute Standard Technology (NIST) material No. 2976, resulting an effective recovery 83 ± 14%. means uncertainties attained each PAH equivalent to those material, corroborating reliability method. A shorter processing time, less use solvents reagents lower extract manipulation produced aligned with green-chemistry guidelines.